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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: NF-κB maintains the stemness of colon cancer cells by downregulating miR-195-5p/497–5p and upregulating MCM2

Fig. 6

miR-195-5p and miR-497-5p target and inhibit the expression of MCM2. a, Differentially expressed genes between tumor samples and normal samples from the GSE75970 dataset. The x-axis represents - log10 p-value and the y-axis represents logFC value. Each dot on the way represents a gene, the red dots represent upregulated genes and the green dots represent downregulated genes. b, Prediction of target genes of miR-497-5p and miR-195-5p. The three circles in the figure represent the predicted results of two miRs and the upregulated genes expressed in the GSE75970 dataset, respectively. The middle part represents the intersection of the three sets of data. c, Interaction and correlation analysis of potential target genes. Each circle in the map represents a gene. Darker color of the circle reflects higher core level of the gene in the whole network map. d, The expression of MCM2 gene in the TCGA colon cancer database. The blue box chart on the left indicates normal samples and the red box chart on the right indicates tumor samples (p < 0.001). e, Specific binding sites of miR-497-5p and miR-195-5p to MCM2 predicted online. f, Luciferase activity at the MCM2 promoter region detected by dual-luciferase reporter gene assay. g, The expression of MCM2, miR-497-5p and miR-195-5p in CCSCs as detected by RT-qPCR. h, The protein expression of MCM 2 in colon cancer cells and CCSCs s detected by Western blot analysis. i, The protein expression of MCM2 in colon cancer and adjacent tissues as detected by Western blot analysis. j, The expression of miR-497-5p, miR-195-5p and MCM2 in colon cancer tissues as detected by RT-qPCR and the corresponding correlation analysis scatter plots (n = 35). Measurement data were expressed as mean ± s.d. Data between two groups were compared using unpaired t-test. Data among multiple groups were compared using one-way analysis of variance and then analyzed with Tukey’s post-hoc test. Cell experiment was repeated three times. * p < 0.05 vs. NC mimic or adjacent tissues. # p < 0.05 vs. NC-inhibitor

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