Fig. 8

Stem-like properties of CCSCs were inhibited by miR-195-5p/497–5p through negative regulation of MCM2. a, Quantitation for the formation ability of tumor microspheres of CCSCs as detected by tumor sphere self-renewal assay. b, Quantitation for colony formation ability of cells as tested by soft agar colony formation assay. c, Protein expression of stem-like properties-related proteins (CD133 and EpCAM) as detected by Western blot analysis. d, Protein expression of stem cell markers (Nanog, Oct-4 and Sox-2) as detected by Western blot analysis. Measurement data were expressed as mean ± s.d. Data among multiple groups were compared using one-way analysis of variance and then analyzed with Tukey’s post-hoc test. Cell experiment was repeated three times. * p < 0.05 vs. si-NC. # p < 0.05 vs. pcDNA-MCM2 + miR-NC