Fig. 2

MiR-103a-3p serve as oncogenic roles and promote glycolysis in CRC cells. a qRT-PCR assay indicating the levels of miR-103a-3p in SW480 and HCT116 cells stably transfected with miR-103a-3p mimics, mimics control, miR-103a-3p inhibitor or inhibitor control. b Transwell and d CCK-8 assays indicating the invasion, migration and proliferation ability of HCT116 cells transfected with empty vector or miR-103a-3p shRNA (sh-miR-103a-3p). c Angiogenesis ability in HCT116 and SW480 cells transfected with vector-1, sh-miR-103a-3p, vector-2, or miR-103a-3p mimics. e The acidity of the cell culture medium was detected in HCT116 and SW480 cells transfected with vector-1, sh-miR-103a-3p, vector-2, or miR-103a-3p mimics under hypoxia. f-g The ECAR and the variations of glycolysis, glycolysis capacity and glycolysis reserve of CRC cells were analyzed by Seahorse XFe 96 Extracellular Flux Analyzer. h-i qRT-PCR assay showing the expression levels of HK2, LDHA, PFK1, PKM1 and HIF1A in HCT116 and SW480 cells transfected with vector-1, sh-miR-103a-3p, vector-2, or miR-103a-3p mimics. *p < 0.05, **p < 0.01, ***p < 0.01