Fig. 7

MiR-103a-3p affects glycolysis in CRC by regulating the Hippo/YAP1/HIF1A axis. a Immunofluorescence staining assay indicating that YAP1 and HIF1A localization was reduced in the nucleus and cytoplasm of HCT116 cells transfected with miR-103a-3p inhibitor under hypoxic conditions, respectively. b-c The regulation of miR-103a-3p-LATS2/SAV1 axis on HIF1A was determined in HCT116 cells transfected with miR-103a-3p inhibitor, miR-103a-3p inhibitor+si-SAV1, miR-103a-3p inhibitor+si-LATS2, or negative control by qRT-PCR. d-e The regulation of miR-103a-3p-YAP1 axis on HIF1A was determined in HCT116 and SW480 cells transfected with miR-103a-3p inhibitor, miR-103a-3p inhibitor+YAP1, or vector by qRT-PCR. f The ECAR and the variations of glycolysis, glycolysis capacity and glycolysis reserve of CRC cells were analyzed by Seahorse XFe 96 Extracellular Flux Analyzer. g The acidity of the cell culture medium was detected in SW480 and HCT116 cells transfected with miR-103a-3p mimics, miR-103a-3p mimics+si-HIF1A, miR-103a-3p inhibitor, or miR-103a-3p inhibitor+HIF1A. h The acidity of the cell culture medium was detected in SW480 and HCT116 cells transfected with miR-103a-3p mimics, miR-103a-3p mimics+si-YAP1, miR-103a-3p inhibitor, or miR-103a-3p inhibitor+YAP1. i Schematic representation of a model depicting the major molecular mechanisms of the miR-103a-3p-LATS2/SAV1-YAP1-HIF1A axis in CRC under hypoxic conditions