Fig. 1

TRPV1 expression in gastric cancer and its correlation with clinical progression. a Transcript levels of TRPV1 detected by qPCR in human primary GC tissues and their adjacent tissues (*P < 0.05, n = 20 patients). b Representative images of immunohistological staining on TRPV1 proteins in GC tissues and adjacent tissues. c Summary data of immunohistological staining of TRPV1 proteins (***P < 0.001, n = 80 patients). d Analysis of survival rates in GC patients with low and high TRPV1 expression levels (**P < 0.01, n = 100 patients). Correlation between TRPV1 and Ki67 (e), VEGFR (f), and E-cadherin (g) expression (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 100 patients). h Western blot analysis of TRPV1 protein levels in a normal human gastric epithelial cell line (GES-1) and five GC cell lines (**P < 0.01 vs. GES-1, n = 3). i QPCR analysis of TRPV1 mRNA levels in GES-1 and nine GC cell lines (**P < 0.01, ***P < 0.001, ****P < 0.0001 vs. GES-1, n = 3). j Immunofluoresence staining images of TRPV1 proteins in GES-1, MKN45 and BGC823 cells. Negative control was not treated with primary antibody against TRPV1. Nuclei were stained in blue with DAPI. The white scale bars on the lower right are 7.5 μm. These images are representative of three independent experiments