Fig. 5
Characterization of EVs from TTLL4plus and control cells. a EVs were isolated by differential centrifugation and analyzed by TEM. Shown are representative images, Bar: 100 nm. b Particle concentration and size distribution were examined by NTA. Mean of three independent experiments is shown. c, d The concentration of Calnexin, Hsc70, Flottilin-1 and Alix in EVs were analyzed by Western blotting. e Quantification of Hsc70, Flottilin-1 and Alix signals in cell lysates and EVs was performed by ImageJ and ratios of signals derived from EV proteins and cell lysates were calculated. Shown are mean ± SD of four different signals