Fig. 7
EVs from TTLL4plus cells increase adhesion on and permeability of hCMEC/D3-cells. a hCMEC/D3-cells were grown to confluence on Transwell cell culture chambers and incubated with EVs from MDA-MB231 or MDA-MB468 control or TTLLplus cells for 16 h. Thereafter, fluorescein isothiocyanate-dextran (MW 40,000) was added and, after 4 h, green fluorescence was measured in the lower reservoir. b hCMEC/D3-cells grown to confluence on chamber slides were incubated with EVs from MDA-MB231 or MDA-MB468 control or TTLLplus cells for 16 h. Then, MDA-MB231 or MDA-MB468 control cells loaded with CellTracker™ Green CMFDA were added and after 4 h, green fluorescent cells were counted. Mean ± SD of eight different determinations are shown