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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: TAM family receptors in conjunction with MAPK signalling are involved in acquired resistance to PI3Kα inhibition in head and neck squamous cell carcinoma

Fig. 2

Genomically-distinct HNSCC cell lines become resistant to PI3K inhibition over time. a Genomic features and IC50 values for Cal33 and 93-VU-147T cell lines. b Phase contrast microscopy images of parental and resistant HNSCC cell lines, with and without 5 μM alpelisib treatment for 24 h. c Dose response curves comparing sensitivity of parental and resistant cell lines over 10 doses of alpelisib. d Colony formation assays comparing tolerance of parental and alpelisib-resistant cell lines to alpelisib treatment over time. Number of colonies was counted and graphed. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, ns = not significant, unpaired Student’s t-test. e Immunoblot for PARP cleavage in Cal33 and 93-VU-147T parental and alpelisib-resistant cells treated with alpelisib (5 μM) for 45 h, or staurosporine (2 μM) for 3 h, as a positive control. Densitometric quantification of each protein relative to α-tubulin is shown below each band

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