Fig. 3

Expression of AXL and TYRO3 is elevated in alpelisib-resistant cells. a Immunoblot with/without 5 μM alpelisib (36 h) in parental and resistant Cal33 and 93-VU-147T cell lines using indicated antibodies. Densitometric quantification of each protein relative to α-tubulin is shown below each band. b Heatmaps displaying RPPA results (top 50% of differentially-expressed proteins for each cell line is shown). Arrows indicate AXL and TYRO3. c Immunoblot showing expression of TAM family RTKs AXL, TYRO3 and MER-TK in parental and alpelisib-resistant HNSCC cell lines. HEK293T cells are included as a positive control for MER-TK expression. Densitometric quantification of each protein relative to α-tubulin is shown below each band