Fig. 5

TYRO3 and AXL modulate sensitivity to alpelisib. a Flow cytometric analysis of AXL and TYRO3 in parental and resistant HNSCC cell lines. Median fluorescence intensity (MFI) was measured and graphed for three biological replicates. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, **** represents p < 0.0001, ns = not significant, unpaired Student’s t-test. b siRNA-mediated knockdown of AXL (siAXL) and TYRO3 (siTYRO3) in Cal33 and 93-VU-147T cells. siCT = scrambled control siRNA, RNAi = RNA interference. ** represents p < 0.01, ns = not significant. One-way ANOVA. Immunoblot of AXL and TYRO3 expression following siRNA-mediated knockdowns is shown below, siCT = scrambled control siRNA. Densitometric quantification of each protein relative to α-tubulin is shown below each band