Fig. 1

LncRNA RP11-757G1.5 expression is upregulated in CRC tissues and is associated with poor prognosis. a Heat-maps of lncRNAs that were differentially expressed between CRC tissues and matched adjacent normal samples. RP11-757G1.5 was the most appropriate lncRNA to select in eight lncRNAs. The color scale shown below illustrates the relative RNA expression levels; red represents high expression, and blue represents low expression. b The expression of RP11-757G1.5 was determined by in situ hybridization at different lymph node metastasis, TNM stages of CRC patients compare with corresponding adjacent normal tissues. Scale bar, 50 μm. c Comparison of RP11-757G1.5 in CRC tissues (n = 64) and normal tissues (n = 56) by RT-qPCR. d-e RP11-757G1.5 expression at different lymph node metastasis (Normal: normal adjacent tissues; N0: no lymph node metastasis; N1: 1 ~ 3 regional lymph node metastasis; N2: ≥4 regional lymph node metastases, n = 18) and TNM stages (n = 15) of CRC patients. f-g Kaplan-Meier survival analysis of the overall survival and disease-free survival in two groups defined by low and high expression of RP11-757G1.5 in patients with CRC. The median expression of RP11-757G1.5 was used as cut-off. p = 0.0047 and p = 0.0129 by Log-rank test. *p < 0.05, **p < 0.01 by Student’s t-test. Data are representative of at least three independent experiments