Fig. 2

Overexpression and stable knockdown of RP11-757G1.5 in HCT-116 and SW480 cells. a Relative expression of RP11-757G1.5 in six CRC cell lines and a colonic epithelial cell. b Fluorescence in situ hybridization (FISH) assay was conducted to determine the subcellular localization of RP11-757G1.5 in HCT-116 and SW480 cells. Nuclei are stained blue (DAPI), and RP11-757G1.5 are stained green. Scale bar, 25 μm. c-d Representative images of HCT-116 and SW480 cells transfected with pcDNA3.1-757G1.5 and sh-757G1.5#1; sh-757G1.5#2, respectively. Scale bar, 25 μm. e-f The validation of overexpression and knockdown efficacy of RP11-757G1.5 in CRC cell lines by RT-qPCR. *p < 0.05, **p < 0.01 by Student’s t-test, Data are representative of at least three independent experiments