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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long noncoding RNA RP11-757G1.5 sponges miR-139-5p and upregulates YAP1 thereby promoting the proliferation and liver, spleen metastasis of colorectal cancer

Fig. 6

RP11-757G1.5 exerts tumor-promoting function in CRC by regulating the miR-139-5p/YAP1 axis. a-d The increased cell proliferation ability in pcDNA3.1-757G1.5 transfected CRC cells was abolished by ectopic miR-139-5p expression or YAP1 knockdown. The cell proliferation ability was measured by a colony formation and Edu proliferation assay. Scale bar, 25 μm. e Western blotting analysis of proliferation-associated antigens in HCT116 cells transfected with miR-139-5p mimic or co-transfected with miR-139-5p mimic and pcDNA3.1-757G1.5. f Western blotting was used to determine the changes of the proliferation-associated antigens in HCT-116 cells transfected with si-YAP1 (siRNA) or co-transfected with si-YAP1 and pcDNA3.1-757G1.5. Tubulin served as a loading control. g-j The increased cell migration and invasion ability in pcDNA3.1-757G1.5 transfected CRC cells were abolished by ectopic miR-139-5p expression or YAP1 knockdown. Cell migration and invasion were measured by wound healing and transwell assays. Scale bar, 25 μm. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test. The data represented from at least three independent experiments

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