Fig. 1

WTX loss correlates with advanced gastric cancer (GC) progression and poor patient prognosis. a IHC staining of WTX in normal (N) stomach mucosa and well-, moderately, and poorly differentiated GC tissues. Scale bars, 50 μm. b IHC staining analysis of WTX expression in GC and adjacent normal mucosal tissues. n = 161. Chi-square test. c Ratio (GC/N) of WTX mRNA expression in 55 GC tissues and adjacent normal mucosal tissues, as determined by RT-PCR. Expression levels were normalized to those of GAPDH. d IHC Staining analysis of WTX expression in GC tissues with or without lymph node metastasis. n = 138. Chi-square test. e IHC staining analysis of WTX expression in GC tissues with different differentiation characteristics. n = 138. Chi-square test. f Kaplan-Meier survival analysis of GC patients with positive or negative WTX expression based on IHC data. g-h Kaplan-Meier survival analyses of GC patients with high or low WTX expression based on KM plotter database and GSE15459 dataset