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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Mir-20a-5p induced WTX deficiency promotes gastric cancer progressions through regulating PI3K/AKT signaling pathway

Fig. 3

WTX inhibits PI3K/AKT/mTOR pathway activation. a The top 20 hits from the KEGG pathway enrichment analysis performed with microarray data for AGS. W and AGS.veh. b A heatmap based on 26 overlapping genes in PI3K/AKT signaling pathway identified from the AGS. W vs AGS.veh comparison. c A heatmap based on 31 overlapping genes in the PI3K/AKT signaling pathway in the GSE34715 dataset. d Western blot analyses of PI3K/AKT/mTOR pathway proteins in the indicated cells. GAPDH is shown as a loading control. e-g The WTX, p-AKT, and p-mTOR IHC staining scores in tumours of orthotopic models. ***P < 0.001, **P < 0.01, *P < 0.05, means±SD, n = 5. Two-tailed Student’s t-test. h The percentage of positively stained Ki-67 cells in tumours of orthotopic models. ***P < 0.001, **P < 0.01, *P < 0.05, means±SD, n = 5. Two-tailed Student’s t-test. i Representative images of H&E staining and WTX, p-AKT, p-mTOR, and Ki-67 IHC staining of tumours from orthotropic models

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