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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Nicotinamide inhibits melanoma in vitro and in vivo

Fig. 4

NAD+, ATP, and ROS levels under NAM treatment. a, b, d, e: NAM treatment increased NAD+ and ATP levels in both A375 and SK-MEL-28 cells (8 × 104 and 1 × 105 in p35 Petri dishes, respectively) after 6 h treatment. c, f: NAM increased ROS levels in A375 and SK-MEL-28 cells. The cells (8 × 104 and 1 × 105 in p35 Petri dishes, respectively) were stained with 2′,7′-dichlorodihydroflurescein diacetate (DCFDA) and then treated with 50 mM NAM for 6 h. All data are expressed as mean ± S.D. of three independent experiments. In panels a, b, d, e one-way ANOVA test as statistical assay computed a p < 0.05. In panels c and f a p < 0.05 was computed, according to Student’s t-test

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