Fig. 5
NAM effects on mouse B16-F10 melanoma cells. a: B16-F10 cells were counted after NAM treatment for 24 and 48 h. B16-F10 cells (8 × 104) were plated in p35 Petri dishes at time 0, in the presence of 10% FBS. Cell number is significantly reduced in a dose-dependent manner. Data are expressed as percentage of untreated cells. b: Quantification of cell death by trypan blue exclusion assay, expressed as absolute numbers. c: ROS levels evaluated by 2′,7′- dichlorodihydrofluorescin diacetate (DCFDA) staining in cells treated with 50 mM NAM for 6 h. d: Representative fields of B16-F10 cells treated with NAM 50 mM for 24 and 48 h are shown (100x magnification). Scale bar = 50 μm. All data are expressed as mean ± S.D. of three independent experiments. In panels a and b one-way ANOVA test computed a p < 0.05. In panel c Student’s t-test computed a p < 0.05