Fig. 7
Antitumor effect and toxicity of NAM administered 5 days per week, i.p. C57BL/6 mice were injected s.c. with 3 × 105 B16-F10 cells. Mice were divided into four groups (n = 4) and treated i.p. with NAM 1000 (grey square), 1500 (open triangle) or 1800 (asterisk) mg/Kg or with saline (ctr, black circle), as indicated, starting from the same day of tumor implant for 5 days per week. a: Tumor growth was monitored twice a week with a digital caliper. Data are reported as the average of the mean tumor diameter of 4 mice per group ± S.E. b: Mouse body weight was monitored twice a week as an indication of NAM toxicity. Data are reported as mean weight of 4 mice per group ± S.E. P-values were calculated by Mann-Whitney U test for independent samples (NS, not significant)