Fig. 2

Knockdown of HDAC3 inhibits CRC cell proliferation and metastasis. a. RT-qPCR detection of HDAC3 mRNA, miR-296-3p and TGIF1 mRNA expression in cells after knockdown of HDAC3; b. Western blot analysis determination of HDAC3 and TGIF1 protein expression in cells after knockdown of HDAC3; c. Western blot analysis determination of TGFβ signaling pathway-related proteins in cells after knockdown of HDAC3; d. MTT assay of cell viability in cells after knockdown of HDAC3; e. EdU assay of cell proliferation after knockdown of HDAC3; f. Flow cytometry of cell cycle distribution after knockdown of HDAC3; g. Flow cytometry of cell apoptosis after knockdown of HDAC3; h. Scratch test of cell migration after knockdown of HDAC3; i. Transwell assay of cell invasion after knockdown of HDAC3; j. RT-qPCR detection of PCNA, ki-67, MMP2 and MMP9 expression in cells after knockdown of HDAC3. N = 3. One-way ANOVA was utilized for comparisons among multiple groups, followed by Tukey’s multiple comparisons test. * represented P < 0.05, ** represented P < 0.01, *** represented P < 0.001. si-NC; HDAC3 siRNA negative control; si-HDAC3; siRNA HDAC3