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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis

Fig. 3

Restored miR-296-3p or depleted TGIF1 suppresses CRC cell proliferation and metastasis. a. RT-qPCR detection of miR-296-3p and TGIF1 mRNA expression in cells after up-regulating miR-296-3p or down-regulating TGIF1; b. Protein bands of TGIF1 protein in cells after up-regulating miR-296-3p or down-regulating TGIF1; c. Comparison of TGIF1 protein expression in cells after up-regulating miR-296-3p or down-regulating TGIF1; d. Analysis of TGFβ signaling pathway-related proteins in cells after up-regulating miR-296-3p or down-regulating TGIF1; e. MTT assay of cell viability after up-regulating miR-296-3p or down-regulating TGIF1; f. EdU assay of cell proliferation after up-regulating miR-296-3p or down-regulating TGIF1; g. Flow cytometry of cell cycle distribution after up-regulating miR-296-3p or down-regulating TGIF1; h. Flow cytometry of cell apoptosis after up-regulating miR-296-3p or down-regulating TGIF1; i. Scratch test of cell migration after up-regulating miR-296-3p or down-regulating TGIF1; j. Transwell assay of cell invasion after up-regulating miR-296-3p or down-regulating TGIF1; k. RT-qPCR of PCNA, ki-67, MMP2 and MMP9 expression after up-regulating miR-296-3p or down-regulating TGIF1. N = 3. One-way ANOVA was utilized for comparisons among multiple groups, followed by Tukey’s multiple comparisons test. * represented P < 0.05, ** represented P < 0.01, *** represented P < 0.001. si-CTR; TGIF1 siRNA negative contro; si-TGIF1: siRNA TGIF1; oe-TGIF1: TGIF1 overexpression vector

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