Fig. 4
From: HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis
HDAC3 up-regulates TGIF1 via miR-296-3p inhibition. a. ChIP-qPCR detection of the recruitment level of HDAC3 in the miR-296-3p promoter; b. ChIP-qPCR detection of the recruitment level of HDAC3 in the miR-296-3p promoter after HDAC3 knockdown; c. The putative binding sites of miR-296-3p and TGIF1 were predicted and mutated for the following assays; d. Dual luciferase reporter gene assay of the targeting relationship between miR-296-3p and TGIF1. N = 3. The comparisons between two groups were analyzed by t test. * represented P < 0.05, ** represented P < 0.01, *** represented P < 0.001. WT: wild-type; MUT: mutant