Table 1 Primer sequences
From: HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis
| Genes | Primer sequences |
|---|---|
| miR-296-3p | F: 5′-GAGGGTTGGGTGGAGGCTCTCC −3′ |
| The reverse primers of miR-296-3p used universal primers | |
| U6 | F: 5′-ATGACACGCAAATTCGTGAAGC-3′ |
| The reverse primers of U6 used universal primers | |
| HDAC3 | F: 5′-GGAGCTGGACACCCTATGAA-3 |
| R: 5′-TATTGGTGGGGCTGACTCTC-3′ | |
| TGIF1 | F: 5′-AGATCTGAATTGTGCCAGTGTTTCTCTTTG-3′ |
| R: 5′-CCATGGCGGCGCTTCAGAGTGAG-3′ | |
| TGF-β | F: 5′-GCAAGTGGACATCAACGGGTTC-3′ |
| R: 5′-CGCACGCAGCAGTTCTTCTC-3′ | |
| Smad2 | F: 5′-CGTCCATCTTGCCATTCACG-3′ |
| R: 5′-CTCAAGCTCATCTAATCGTCCTG-3’ | |
| Smad3 | F: 5′-GGACGCAGGTTCTCCAAAC-3’ |
| R: 5′-CGGCTCGCAGTAGGTAA-3’ | |
| PCNA | F: 5′-CGGATACCTTGGCGCTAGTA-3’ |
| R: 5′-TCACTCCGTCTTTTGCACAG-3’ | |
| Ki-67 | F: 5′-GAGAGCTCCCAGCCTAAGGT-3’ |
| R: 5′-TGCACACCTCTTGACACTCC-3’ | |
| MMP2 | F: 5′-CCGTCGCCCATCATCAAGTT-3’ |
| R: 5′-CTGTCTGGGGCAGTCCAAAG-3’ | |
| MMP9 | F: 5′-CATTTCGACGATGACGAGTTGT-3’ |
| R: 5′-CGGGTGTAGAGTCTCTCGC-3’ | |
| GAPDH | F: 5′-GACAACAGCCTCAAGATCATCAG-3’ |
| R: 5′-GTGGCAGTGATGGCATGGA-3’ |