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Table 1 Primer sequences

From: HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis

Genes Primer sequences
miR-296-3p F: 5′-GAGGGTTGGGTGGAGGCTCTCC −3′
The reverse primers of miR-296-3p used universal primers
U6 F: 5′-ATGACACGCAAATTCGTGAAGC-3′
The reverse primers of U6 used universal primers
HDAC3 F: 5′-GGAGCTGGACACCCTATGAA-3
R: 5′-TATTGGTGGGGCTGACTCTC-3′
TGIF1 F: 5′-AGATCTGAATTGTGCCAGTGTTTCTCTTTG-3′
R: 5′-CCATGGCGGCGCTTCAGAGTGAG-3′
TGF-β F: 5′-GCAAGTGGACATCAACGGGTTC-3′
R: 5′-CGCACGCAGCAGTTCTTCTC-3′
Smad2 F: 5′-CGTCCATCTTGCCATTCACG-3′
R: 5′-CTCAAGCTCATCTAATCGTCCTG-3’
Smad3 F: 5′-GGACGCAGGTTCTCCAAAC-3’
R: 5′-CGGCTCGCAGTAGGTAA-3’
PCNA F: 5′-CGGATACCTTGGCGCTAGTA-3’
R: 5′-TCACTCCGTCTTTTGCACAG-3’
Ki-67 F: 5′-GAGAGCTCCCAGCCTAAGGT-3’
R: 5′-TGCACACCTCTTGACACTCC-3’
MMP2 F: 5′-CCGTCGCCCATCATCAAGTT-3’
R: 5′-CTGTCTGGGGCAGTCCAAAG-3’
MMP9 F: 5′-CATTTCGACGATGACGAGTTGT-3’
R: 5′-CGGGTGTAGAGTCTCTCGC-3’
GAPDH F: 5′-GACAACAGCCTCAAGATCATCAG-3’
R: 5′-GTGGCAGTGATGGCATGGA-3’
  1. F forward, R reverse, miR-296-3p microRNA-296-3p, HDAC3 Histone Deacetylase 3, TGIF1 thymine-guanine-interacting factor1, TGF-β transforming growth factor-β, Smad2 mothers against decapentaplegic homolog 2, Smad3 mothers against decapentaplegic homolog 3, PCNA proliferating cell nuclear antigen, MMP2 matrix metalloproteinase 2, MMP9 matrix metalloproteinase 9, GAPDH glyceraldehyde-3-phosphate dehydrogenase