Fig. 3

JM1-24-3 binds to MUC18 on melanoma cells and stimulates downstream signaling pathways in RPPA and computational models. a Heat maps illustrating the differences in the expression of proteins in WM266-4 cell lysates with 1 h/6 h/no treatment with JM1-24-3 as analyzed by RPPA. b Fold changes for up- or down-regulated proteins in RPPA as analyzed with the IPA software is shown. c WM266-4 cells treated with JM1-24-3/irrelevant mAb/PBS at different time points (30 min-24 h) showed by WB that p-AKT (Ser473) and p-mTOR (Ser2448) had time-dependent reduction in phosphorylation until 6 h, while both total AKT and mTOR remained unchanged and β-actin served as loading control. d Structural models showing the conformation of the MUC18 epitope, marked as pink, blue and red moieties, and relative binding of the heavy-chain, light-chain Fv peptides of JM1-24-3 to the flank and near side of the “bent” conformation compared to binding of the single-chain variable fragment (scFv) of JM1-24-3 to the top surface of MUC18 molecule when in its “extended” conformation