Fig. 5

Linc00337 is a direct target of E2F1. a, western blot analysis of E2F1 protein levels in the indicated cell lines. b, western blot analysis of E2F1 protein levels in the indicated stably-transfected cell lines. c, qRT-PCR analysis of Linc00337 in stably-transfected PANC1 (left) and AsPC1 (right) cells. d, schematic diagram of E2F1 binding sites in the Linc00337 gene promoter. e and f, barplots depicting the regulation of Linc00337 transcription by E2F1 in PANC1 (e) and AsPC1(f) cells. g, ChIP-qPCR analysis of E2F1 occupancy at the Linc00337 promoter in PANC1 cells. In (a-c and e-f), data represent the mean ± SD of three independent experiments