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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma

Fig. 6

Linc00337 binds to E2F1 and acts as a E2F1 coactivitor. a, RNA FISH was carried out to determine the subcellular localization of Linc00337 in PANC1 cells. b, in vitro-transcribed biotin-labeled Linc00337 and its antisense RNA (top) and RNA pulldown assays showing that Linc00337 pulled down E2F1 and its partner DP1, but not GAPDH in PANC1 cells (bottom). c, Flag-E2F1 RIP assays suggesting that Linc00337 could be enriched by E2F1 in PANC1 cells. d, Stably-transfected PANC1 (left) and AsPC1 (right) cells were used to determine the transcriptional activity of E2F1. e, qRT-PCR analysis of E2F1 targets in indicated stably-transfected PANC1 cells. f, ChIRP-qRT-PCR detection of Linc00337 occupancy at the indicated E2F1 target loci. Data represent the mean ± SD of three independent experiments

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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