# qRT-PCR primers (5′-3′) | ||
 | Forward | Reverse |
337 V1 | CTCTGATCTGTCCACCCTCG | TTCCTGGGGTTTGTGTTCGG |
337 V2 | GCGAATCATTTGAGTAGAGA | CAAGTGAAGTCAGGATCACA |
337 V1 + V3 | AGTTGCTGGAGTTGCCGAAT | TCTCCGTGTGTGTGTTTCCC |
18S | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG |
JUNB | ACAAACTCCTGAAACCGAGCC | CGAGCCCTGACCAGAAAAGTA |
JUND | TCATCATCCAGTCCAACGGG | TTCTGCTTGTGTAAATCCTCCAG |
CCNB1 | AATAAGGCGAAGATCAACATGGC | TTTGTTACCAATGTCCCCAAGAG |
CCNB2 | CCGACGGTGTCCAGTGATTT | TGTTGTTTTGGTGGGTTGAACT |
MYC | GGCTCCTGGCAAAAGGTCA | CTGCGTAGTTGTGCTGATGT |
ChIRP used primers (5′-3′) | ||
CCNB1 | TGTGACCCTGGCAAAGTCAT | CAAGAGTGTGCGTTGCCAAT |
JUNB | ATGCGTACCCCGAGGTCCTTTGA | AGCCTGAGCCACACGCCTTTATA |
MYC | TTTATAATGCGAGGGTCTGG | TATTCGCTCCGGATCTCCCTT |
FOXM1 | GAGCTTTGAAAAGGGGAGCA | ACCGGAGCTTTCAGTTTGTT |
shRNA target sequenses (5′-3′) | ||
337 sh#1 | CCTCCCAAAGTGCTGAGATTA | Â |
337 sh#2 | CCTCCCAAAGTGCTGAGATTA | Â |
337 sh#3 | GTACCACTTTATTTTATTTTA | Â |
E2F1 shRNA | AGCTGGACCACCTGATGAATA | Â |
ChIP-qRT-PCR primers | Â | |
Forward | Reverse | |
337BS1 | CCAAGTAGCTAGAATTACAGC | TTTAATCCCAGCACTTTGGGA |
337BS2 | TGCGCACTGTGCCGCCGATGC | CTCCTGAGTTTGGGACTAAGT |
U1 | ATACTTACCTGGCAGGGGA | AGGGGAAAGCGCGAACGCAG |
MYC | AGATCCTCTCTCGCTAATCT | ATACTCAGCGCGATCCCTCC |