Fig. 2

SETDB1 promotes cell growth, and apoptosis in vitro and in vivo. a mRNA level of SETDB1 in indicated cells transfected with SETDB1 plasmid or control vector. b Western blotting of SETDB1 in indicated cells transfected with SETDB1 plasmid or control vector. c CCK-8 of indicated cells transfected with SETDB1 plasmid or control vector. d Colony formation of indicated cells transfected with SETDB1 plasmid or control vector. e Western blotting of indicated proteins in U251 cells transfected with SETDB1 plasmid or control vector treated with 500 nM staurosporine (STS) for 24 h. f Ectopic expression of SETDB1 accelerated growth of U251 xenografts in nude mice (n = 6) as compared to controls. g Western blotting of ectopic expression of SETDB1 in tumors from U251-SETDB1 groups. h Representative images of Ki67-positive cells in vector and SETDB1 transfected tumors. Scale bar: 50 μm. Results were expressed as means ± SD of 3 independent experiments. **, P < 0.01