Fig. 3

SETDB1 promotes invasion, migration and EMT of glioblastoma cells. a Representative images of matrigel invasion assay revealed that ectopic expression of SETDB1 promoted glioblastoma cells invasion. Scale bar: 100 μm. b Representative images of wound-healing assay indicated that ectopic expression of SETDB1 promoted cell migration in glioblastoma cells. Scale bar: 100 μm. c Western blotting of mesenchymal markers (Slug and Vimentin) and epithelial markers (E-cadherin) in cells transfected with SETDB1 or control plasmid. Scale bar: 100 μm. d Representative images of matrigel invasion assay revealed that downregulation of SETDB1 suppressed glioblastoma cells invasion. Scale bar: 100 μm. e Representative images of wound-healing assay indicated that downregulation of SETDB1 suppressed cell migration in glioblastoma cells. f Western blotting of mesenchymal markers (Slug and Vimentin) and epithelial markers (E-cadherin) in cells transfected with stable shRNA against SETDB1. Results were expressed as means ± SD of 3 independent experiments. **, P < 0.01