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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: SETDB1 promotes glioblastoma growth via CSF-1-dependent macrophage recruitment by activating the AKT/mTOR signaling pathway

Fig. 4

SETDB1 regulates CSF-1 level in glioblastoma cells. a Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U87 cells with SETDB1 overexpression. b Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U251 cells with SETDB1 overexpression. c Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U87 cells with SETDB1 knockdown. d Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U251 cells with SETDB1 knockdown. e Enzyme-linked immunosorbent assay (ELISA) analysis of CSF-1 concentration in the supernatants of cultured glioblastoma cells with SETDB1 overexpression. f Enzyme-linked immunosorbent assay (ELISA) analysis of CSF-1 concentration in the supernatants of cultured glioblastoma cells with SETDB1 knockdown. g TCGA database indicated the correlation of CD163 and CSF-1 in glioblastoma tissues. h TCGA database indicated the correlation of SETDB1 and CSF-1 in glioblastoma tissues. Results were expressed as means ± SD of 3 independent experiments. **, P < 0.01

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