Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: SETDB1 promotes glioblastoma growth via CSF-1-dependent macrophage recruitment by activating the AKT/mTOR signaling pathway

Fig. 6

SETDB1 promotes macrophage recruitment and polarization. a Transwell migration assay of macrophage by CM from indicated U87 cells. b Real-time PCR for the expression levels of CD68 and CD163 in macrophages treated with CM from U87 cells as indicated. c Flow cytometry analysis for the expression levels of CD163 in macrophages treated with CM from glioblastoma cells as indicated. d Real-time PCR for the mRNA expression of tumor-associated macrophage (TAM) characteristic cytokines in macrophages treated with CM from glioblastoma cells as indicated. e Enzyme-linked immunosorbent assay (ELISA) for the secretion of tumor-associated macrophage (TAM) characteristic cytokines in macrophages treated with CM from U87 cells as indicated. f Real-time PCR for the mRNA expression of M1-related cytokines in macrophages treated with CM from U87 cells as indicated. g Enzyme-linked immunosorbent assay (ELISA) for the secretion of M1-related cytokines in macrophages treated with CM from U87 cells as indicated. Results were expressed as means ± SD of 3 independent experiments. **, P < 0.01

Back to article page