Fig. 6

miR-96 impairs m6A modification of MYC by targeting AMPKα2. a: The protein expression of FTO and MYC in CRC cells co-transfected with miR-96 mimic/NC mimic and oe-NC/oe-AMPKα2 measured by Western blot analysis. b: The m6A modification level of MYC in CRC cells co-transfected with miR-96 mimic/NC mimic and oe-NC/oe-AMPKα2 assessed by Me-RIP. c: The binding relationship between FTO and MYC mRNA examined by PAR-CLIP assay; d: Expression of MYC following the transfection of si-AMPKα2 detected by RT-qPCR. ^*p < 0.05. Measurement data (mean ± standard deviation) among multiple groups were compared by one-way ANOVA with Tukey’s post hoc tests. The cell experiment was independently repeated for three times