Table 1 Primer sequences for RT-qPCR
Gene | Primer sequence |
|---|---|
miR-96 | F: 5’-TTGGGTGAAATATATTGTGCGTCTC-3’ |
R: 5’-AGCCGAAGTGAGCCACTGAA-3’ | |
U6 | F: 5’-GCACCGTCAAGGCTGAGAAC-3’ |
R: 5’-AGCCGAAGTGAGCCACTGAA-3’ | |
AMPKα2 | F: 5’-GGGACCTGAAACCAGAGAACG-3’ |
R: 5’-ACAGAGGAGGGCATAGAGGATG-3’ | |
FTO | F: 5’-TGAAGGTAGCGTGGGACATAGA-3’ |
R: 5’-GGTGAAAAGCCAGCCAGAAC-3’ | |
MYC | F: 5’-TTCGGGTAGTGGAAAACCAG-3’ |
R: 5’-AGTAGAAATACGGCTGCACC-3’ | |
MYC-m6A | F: 5’-GCATACATCCTGTCCGTCCA-3’ |
R: 5’-GTCGTTTCCGCAACAAGTCCC-3’ | |
GAPDH | F: 5’-GCACCGTCAAGGCTGAGAAC-3’ |
R: 5’-TGGTGAAGACGCCAGTGGA-3’ |