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Table 1 Primer sequences for RT-qPCR

From: microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis

Gene Primer sequence
miR-96 F: 5’-TTGGGTGAAATATATTGTGCGTCTC-3’
R: 5’-AGCCGAAGTGAGCCACTGAA-3’
U6 F: 5’-GCACCGTCAAGGCTGAGAAC-3’
R: 5’-AGCCGAAGTGAGCCACTGAA-3’
AMPKα2 F: 5’-GGGACCTGAAACCAGAGAACG-3’
R: 5’-ACAGAGGAGGGCATAGAGGATG-3’
FTO F: 5’-TGAAGGTAGCGTGGGACATAGA-3’
R: 5’-GGTGAAAAGCCAGCCAGAAC-3’
MYC F: 5’-TTCGGGTAGTGGAAAACCAG-3’
R: 5’-AGTAGAAATACGGCTGCACC-3’
MYC-m6A F: 5’-GCATACATCCTGTCCGTCCA-3’
R: 5’-GTCGTTTCCGCAACAAGTCCC-3’
GAPDH F: 5’-GCACCGTCAAGGCTGAGAAC-3’
R: 5’-TGGTGAAGACGCCAGTGGA-3’
  1. Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; F, forward, R, reverse; miR-96, microRNA-96; m6A, N6-methyladenosine; AMPKα2, AMP-activated protein kinase-alpha2; FTO, fat mass and obesity-associated; MYC, c-myc proto-oncogene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase
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