Fig. 2

KDM5A stimulates PCa cell proliferation, migration and invasion while reducing cell apoptosis. a and b, The silencing efficiency of KDM5A in PC3 cells detected by RT-qPCR and Western blot analysis. c, KDM5A mRNA expression in oe-KDM5A-transfected cells detected by RT-qPCR. d, Western blots of KDM5A protein in oe-KDM5A-transfected cells. e, PC3 cell proliferation following oe- or sh-KDM5A transfection measured by colony formation assay. f, PC3 cell apoptosis following oe- or sh-KDM5A transfection measured by flow cytometry. g, PC3 cell invasion following oe- or sh-KDM5A transfection measured by Transwell assay. Scale bar = 50 μm. h, PC3 cell migration following oe- or sh-KDM5A transfection measured by Scratch test. * p < 0.05 vs. cells transfected with sh-NC or oe-NC. Data in panel A, B and E-H were analyzed by one-way ANOVA with Tukey’s post hoc test, and data in panel C and D were analyzed by unpaired t-test