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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Activation of the KDM5A/miRNA-495/YTHDF2/m6A-MOB3B axis facilitates prostate cancer progression

Fig. 6

YTHDF2 recognizes the m6A modification of MOB3B mRNA, evokes mRNA degradation and suppresses MOB3B expression. a, MOB3B mRNA expression in cancer and adjacent normal tissues of 75 patients detected by RT-qPCR. b, Correlation between MOB3B and YTHDF2 in cancer and adjacent normal tissues of 75 patients. c, Western blot analysis of MOB3B protein in cancer and adjacent normal tissues of 75 patients. d, m6A modification level of MOB3B in cancer and adjacent normal tissues assessed using Me-RIP. e, The binding of YTHDF2 to the MOB3B mRNA in cancer and adjacent normal tissues confirmed by PAR-CLIP assay. f, MOB3B mRNA expression in oe-YTHDF2-transfected PC3 cells detected by RT-qPCR. g, Western blot analysis of MOB3B protein in oe-YTHDF2-transfected PC3 cells. h, m6A modification in MOB3B in oe-YTHDF2-transfected PC3 cells assessed using Me-RIP. i, The binding of YTHDF2 to the MOB3B mRNA in oe-YTHDF2-transfected PC3 cells confirmed by PAR-CLIP assay. * p < 0.05 vs. adjacent normal tissues or cells transfected with oe-NC. Data in panel A and C-E were analyzed by paired t-test and those in panel F-I were analyzed by unpaired t-test

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