Fig. 10

Validation of the effect of doxorubicin therapy using RT-PCR technique on selected genes related to apoptosis processes. Relative gene expression levels are shown for IL-6, CLU, JUNB, TNFSF10 in treated and untreated group, between P12 and P24 versus P0 on Hs578T and MDA_MB-231 cell lines. The data were normalized to GAPDH and B2M using ΔΔCT method. Data are presented as mean ± SD using two-sided t-test