Fig. 2

HMGB3was identified as a new transcriptional factor of hTERT in cervical cancer radioresistant cells. a Differential gene expression between radioresistant and parental cervical cancer cells from RNA-Seq. b A heatmap showing 42 significantly up-regulated genes in the RR SiHa and RR HeLa. c A demonstration map of hTERT promoter probe with a biotin from − 1645 to + 15. d HMGB3 was identified as a new transcriptional factor of hTERT by streptavidin-agarose and LC/MS. e Biotin-streptavidin pulldown was perform to further verify the binding of HMGB3 on the hTERT promoter. f Cervical cancer cells were transfected with HMGB3-overexpressing plasmids or HMGB3 specific siRNAs. The expression of HMGB3 and hTERT were detected by western blot. g SiHa was transfected with HMGB3 specific siRNAs, and then the binding ability of HMGB3 on hTERT promoter (− 1645 to + 15) was detected by pulldown assay. h SiHa and HeLa S3 cells were transfected with HMGB3 specific siRNAs, and the activity of different fragments of hTERT promoter was detected by Luciferase Reporter assay. i CHIP assay was used to verify the binding of HMGB3 and H3K4me3 on region − 902 to − 321 of hTERT promoter