Fig. 3

Knockdown of HMGB3 increased the susceptibility to radiotherapy in cervical cancer cells and the xenograft mouse model Cervical cancer cells were transfected with HMGB3-overexpressing plasmids (a) or HMGB3 specific siRNAs (b) and then exposed to 4Gy radiation. At 24 h, colony formation assay was performed.SiHa and HeLa S3 were transfected with HMGB3 specific siRNAs and then exposed to 4Gy radiation. At 24 h, comet assay was performed to detect DNA damage (c), and FACS analysis was performed to detect cell apoptosis(d). e SiHa was infected by lentivirus-HMGB3 shRNA. Cells were implanted into left armpit of the nude mice. Mice were sacrificed on day 15 and tumors were removed and photographed. f Recorded the tumor volume every two days during the course of the experiment.Tumor volume (g) and and weight (h) was measured and recorded. i Immunohistochemical staining for HMGB3, ki67, hTERT and γH2AX. jThe expression of HMGB3, hTERT and γH2AX proteins in mice tumor tissues were detected by Western blot