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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Targeting HMGB3/hTERT axis for radioresistance in cervical cancer

Fig. 4

HMGB3 promoted cervical cancer radioresistance by activating hTERT. SiHa was stably silenced of HMGB3 followed by hTERT overexpression. The level of HMGB3 and hTERT were checked by western blot (a) and PCR (b). c SiHa was stably silenced of HMGB3 followed by hTERT overexpression. The colony formation were detected after 4 Gy radiation. d Quantification of C. SiHa was stably silenced of HMGB3 followed by hTERT overexpression. The cells were then exposed to 4 Gy radiation. Comet assay was performed after 24 h (e). The expression level of γH2AXwas checked by western blot (f). Stable SiHa was left armpit of the nude mice. Mice were sacrificed on day 15, and tumors were removed and photographed (g).Recorded the tumor volume every two days during the course of the experiment (h). Tumor volume was measured and recorded (i). j Immunohistochemical staining for HMGB3, hTERT and γH2AX in the mice tumor tissue

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