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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Ascorbic acid (vitamin C) synergistically enhances the therapeutic effect of targeted therapy in chronic lymphocytic leukemia

Fig. 2

The JVM3 CLL cell line is less-sensitive to AA’s effects than the OSU-CLL cell line. a: Viability of OSU-CLL and JVM3 cells, assessed in a flow cytometry assay using annexin-V-APC and 7-AAD staining after treatment with vehicle or 250 μM AA. b: Viability of JVM3 and OSU-CLL cell lines after treatment with increased concentrations of AA (**: p < 0.01, ***: p < 0.001 vs. OSU-CLL at the same concentrations; n = 5). c, d: Mitochondrial ROS levels, as recorded in a MitoSox flow cytometry assay in OSU-CLL (c) and JVM3 cell lines (d) treated for 6 h with 250 μM AA and expressed as the fold change vs. vehicle (*: p < 0.05 vs. vehicle; n = 7). Data are presented as the fold change in mean ± SEM fluorescence intensity (MFI) for the MitoSox dye. e, f: The GSH/GSSG ratio in OSU-CLL (e) and JVM3 (f) cell lines was assessed after 2 h of treatment with 250 μM AA or vehicle, *: p < 0.05, **: p < 0.01 vs. vehicle (n = 3)

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