Fig. 8

MYLK-AS1/miR-424-5p/E2F7 axis positively regulates HCC metastasis, invasion, and angiogenesis via the VEGFR-2 signaling pathway. a E2F7, VEGFR-2, p-VEGFR-2, PLC-λ, p-PLC-λ, Erk1/2, p-Erk1/2, P38, p-P38, FAK, p-FAK, Src, p-Src, Akt, and p-Akt protein expression in Hep-G2 or MHCC-97H cells divided in the following groups: Control, shRNA-NC, shMYLK-AS1-2, NC, and MYLK-AS1. NC represents a blank plasmid control, and MYLK-AS1 is a group overexpressing this lncRNA. The numbers represent the quantification of the relative protein amount. GAPDH was used as the loading control. VEGFR-2 and p-VEGFR-2 protein expression detected after 7-day-co-culture of HUVECs with Hep-G2 or MHCC-97H cells divided in the same groups as above. GAPDH was used as the loading control. b Schematic diagram of the regulatory mechanism of MYLK-AS1/miR-424-5p/E2F7 axis in the promotion of HCC proliferation, metastasis and angiogenesis