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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Unveiling role of sphingosine-1-phosphate receptor 2 as a brake of epithelial stem cell proliferation and a tumor suppressor in colorectal cancer

Fig. 2

The functional role of S1PR2 in a colitis-associated colon cancer model. Changes in (a) body weight, (b) disease activity index, and (c) histological score of S1PR2−/− (n = 12) and S1PR2+/+ (n = 10) mice after induction of colitis-associated colon cancer by a single AOM injection, followed by three complete oral cycles of 2,5% DSS. d Tumor incidence was evaluated macroscopically on day 49. e Representative endoscopic (up panel) and histological images (middle panel) of the intestinal mucosa of S1PR2−/− and S1PR2+/+ treated and untreated mice on day 49. Immunohistochemical staining for the expression of β-catenin (low panel) was performed on formalin-fixed material. f Endoscopic and histological absolute counting of the tumors per mouse. g Dimensions (d) in millimeters (mm) of high-grade adenoma (HGA) and carcinomas lesions in S1PR2and S1PR2+/+ mice. h Immunodetection of BrdU in untreated and AOM/DSS treated mice on day 49 and reported as % of nuclear immunoreaction. The images were acquired by the DotSlide system at 20x objective. *p < 0.05; ** p < 0.01; and ***p < 0.001 by Mann-Whitney test. Data are representative of 2 experiments

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