Fig. 3

Loss of S1PR2 an early event in the intestinal tumorigenesis. a Tumor load in S1PR2^−/−/Apc^min/+ and S1PR2^+/+/Apc^min/+ mice at 21 weeks of age. b Macroscopic examination and quantification of colonic tumors in mice (red dotted line show the tumors). c Size and d histological classification as low (LGA) and high (HGA)-grade adenomas and carcinomas of colonic lesions. e Immunodetection of Ki67 in the small intestine and colon of mice. The percentage of positive Ki67 cells for crypt was counted in 40 fields of view. Mean ± SD, n = 5; *p < 0.05; ** p < 0.01 by unpaired parametric t-test. f-g Graphs are reporting the number and histological classification of tumors in Apc^min/+ mice after 5 weeks of oral administration of JTE013 or vehicle. Mean ± SD, n = 4, p = 0.049 by unpaired parametric t-test. h Immunostaining of S1PR2 in S1PR2^+/+/Apc^min/+ mice and in human intestinal adenomas. The images were acquired by the DotSlide system at 20x objective. i Western blot analysis (left panel) and densitometric analysis (right panel) of S1PR2 in human adenomas (n = 5) and normal colonic mucosa (n = 5) samples. Protein levels were normalized on the β-actin expression. Significance was evaluated by the Mann-Witney test *p < 0.05 and **p < 0.01