Fig. 4

Evodiamine inhibits the inhibition of IFN-γ-induced PD-L1 expression in H1650 and H1975. a-b H650 and H1975 cells were pretreated with DMSO, evodiamine (8 and 16 μM) for 1 h and then treated with IFN-γ for 24 h. MUCI-1-C and PD-L1mRNA expression were determined by quantitative RT-PCR. c-f The cell surface PD-L1 expression on H650 and H1975 is determined using flow cytometry. The column charts below show the quantitative data of relative PD-L1 membrane protein expression. g-h pre-treatment of H1650 and H1975 cells with 8–16 μM evodiamine, evodiamine can decrease the IFN-γ-induced PD-L1 protein expression in H1650 and H1975 cells. The results were presented in three independent experiments with the mean ± S.D. (n = 3,*P < 0.05; **P < 0.01; ***P < 0.001) a: isotryposin control, b: control, c: 8 μM evodiamine, d:16 μM evodiamine, e: IFN-γ (10 ng/ml), f: 8 μM evodiamine +IFN-γ (10 ng/ml), g: 16 μM evodiamine++IFN-γ (10 ng/ml)