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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Gallbladder cancer-associated fibroblasts promote vasculogenic mimicry formation and tumor growth in gallbladder cancer via upregulating the expression of NOX4, a poor prognosis factor, through IL-6-JAK-STAT3 signal pathway

Fig. 5

Verification of NOX4 expression during GCAFs-trggered VM formation in vitro and in vivo. A, in vitro. (a1) qRT-PCR in GCAFs/NFs. NOX4 mRNA expression in GCAFs was significantly upregulated compared to NFs (*P < 0.05). (a2) qRT-PCR in 3-D matrices. NOX4 mRNA expression in 3-D matrices of GBC cells (GBS-SD, SGC-996, OCUG-1) + GCAFs co-culture was significantly higher than that of alone GBC cells culture (all *P < 0.05). (a3) Western blotting in 3-D matrices. The expression of NOX4 protein in 3-D matrices of GBC cells+GCAFs co-culture was significantly upregulated compared with GBC cell+NFs co-culture and alone GBC cells culture (all *P < 0.05). (a4) IHC (× 200) in 3-D matrices. The expression of NOX4 protein in 3-D matrices of GBC-SD + GCAFs co-culture was significantly higher than that of GBC-SD + NFs co-culture or alone GBC-SD culture (all *P < 0.05), but no statistical difference was observed between GBC-SD + NFs group and GBC-SD group (P > 0.05). B, in vivo. (b1) IHC (× 200); (b2) western blotting. The expression of NOX4 protein in GBC-SD + GCAFs xenografts was significantly higher than that of GBC-SD xenografts (all *P < 0.05). (b3) qRT-PCR. The expression NOX4 mRNA in GBC-SD + GCAFs xenografts was significantly upregulated compared with GBC-SD xenografts (*P < 0.05)

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