Fig. 1

Lycorine hydrochloride inhibits gastric cancer cells growth and tumorigenesis. a Viability of MKN-45 and SGC-7901 cells after treatment with 10, 20, and 40 μM LH. DMSO was used as control. b BrdU-positive MKN-45 and SGC-7901 cells after treatment with 20 μM LH for 48 h. DMSO was used as control. The histogram demonstrated the results of the quantification of the number of BrdU-positive cells in MKN-45 and SGC-7901 cells. c Cell cycle of MKN-45 and SGC-7901 cells treated with 20 μM LH for 48 h were analyzed by flow cytometry. DMSO was used as control. Percentage indicated MKN-45 and SGC-7901 cells at different phase. d The expression of CDK1 and CDK2 in gastric cancer cells treated with different concentration of LH (0, 10, 20, 40 μM) for 48 h. Tubulin was used as internal reference. e, f Tumor volume and weight of indicated mice. DMSO and empty vector were used as control. Scale bar =0.5 cm. g IHC of Ki67 in indicated tumors. Scale bar = 20 μm. Gray value of IHC positive signal in panel was quantified. All data were analyzed by unpaired Student’s t-tests and were showed as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001