Fig. 3

Down-regulated circ_001287 contributes to repressed proliferation, migration and invasion abilities in RCC cells. The 786-O cells were transfected with si-NC, si-circ_001287-1, or si-circ_001287-2. a, Expression of circ_001287 in 786-O cells measured using RT-qPCR. b, Expression of linear RNA_001287 in 786-O cells detected using RT-qPCR. c, Viability of 786-O cells measured using CCK-8 assay. d, Cell cycle distribution of 786-O cells determined using flow cytometry. e, Image (× 200) of migration of 786-O cells measured using Transwell assay. f, Quantification analysis of migration ability of 786-O cells measured using Transwell assay. g, Image (× 200) of invasion of 786-O cells evaluated using Transwell assay. h, Quantification analysis of invasion ability of 786-O cells evaluated using Transwell assay. i, The 786-O cell apoptosis assessed using flow cytometry. j, PCNA, KI67, MMP2, MMP9, Cyclin D1, Cyclin E, Caspase-3, and Caspase-9 protein expression in 786-O cells measured using western blot analysis. ^*p < 0.05 vs. 786-O cells treated with si-NC. The data were measurement data and described as mean ± standard deviation. Data among multiple groups were compared with one-way ANOVA, followed by Tukey’s post hoc test. The cell experiment was repeated 3 times. circRNA, circular RNA; RT-qPCR, reverse transcription quantitative polymerase chain reaction; CCK-8, cell counting kit-8