Fig. 6

circ_001287 modulates miR-144-targeted CEP55 and thus hinders the progression of RCC. a, Binding sites between miR-144 and CEP55 predicted through a bioinformatics website. b, Targeting relationship between miR-144 and CEP55 tested by dual-luciferase reporter gene assay. c, Expression of CEP55 in RCC tissues and adjacent normal tissues measured using RT-qPCR (n = 77). d, Expression of CEP55 in RCC tissues and adjacent normal tissues measured using immunohistochemistry (× 200). e, Correlation analysis of circ_001287 and CEP55. f, Correlation analysis of miR-144 and CEP55. The 786-O cells were treated with oe-NC, oe-CEP55, or si-circ_001287 + oe-CEP55. g, mRNA expression of CEP55 in 786-O cells determined using RT-qPCR. h, Protein expression of CEP55 in 786-O cells tested using western blot analysis. i, Viability of 786-O cells measured using CCK-8 assay. j, Cell cycle distribution measured using flow cytometry. k, Image (× 200) of migration of 786-O cells measured using Transwell assay. l, Quantification analysis of migration ability of 786-O cells measured using Transwell assay. m, Image (× 200) of invasion of 786-O cells evaluated using Transwell assay. n, Quantification analysis of invasion ability of 786-O cells evaluated using Transwell assay. o. Apoptotic rate in 786-O cell analyzed using flow cytometry. p, PCNA, KI67, MMP2, MMP9, Cyclin D1, Cyclin E, Caspase-3, and Caspase-9 protein expression in 786-O cells measured using western blot analysis. ^*p < 0.05 vs. adjacent normal tissues or 786-O cells treated with oe-NC; # p < 0.05 vs. the 786-O cells treated with oe-CEP55. The data were measurement data and described as mean ± standard deviation. Paired t-test was used for data comparison between RCC tissues and adjacent normal tissues. Unpaired t-test was used for analysis of data between the other two groups. Data among groups were compared with one-way ANOVA, followed by Tukey’s post hoc test. The data at different time points among different groups were analyzed by the repeated-measures ANOVA and Bonferroni’s post hoc test. The cell experiment was repeated 3 times. miR-144, microRNA-144; CEP55, centrosomal protein 55; RT-qPCR, reverse transcription quantitative polymerase chain reaction; circRNA, circular RNA; mRNA, messenger RNA; CCK-8, cell counting kit-8