Fig. 7

Depleted circ_001287 reduces miR-144-mediated CEP55 expression to inhibit xenograft tumorigenesis in nude mice. Nude mice were injected with 786-O cells transfected with si-NC, si-circ_001287, miR-144 inhibitor, oe-CEP55, si-circ_001287 + miR-144 inhibitor, or si-circ_001287 + oe-CEP55. a, Representative images of xenograft tumors in nude mice. b, Tumor volume in mice. c, Tumor weight in mice. d, The expression of circRNA-001287 and miR-144 in tumor samples determined with RT-qPCR. e, Gray value of CEP55 protein bands in mice evaluated by western blot analysis. f, Protein expression of CEP55 in nude mice measured by western blot analysis. ^*p < 0.05 vs. mice injected with si-NC-transfected 786-O cells. Statistical data were measurement data and described as mean ± standard deviation. Data among multiple groups were compared with one-way ANOVA, along with Tukey’s post hoc test. The data at different time points among different groups were analyzed by the repeated-measures ANOVA and Bonferroni’s post hoc test (n = 10). CEP55, centrosomal protein 55