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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Extracellular vesicular Wnt7b mediates HPV E6-induced cervical cancer angiogenesis by activating the β-catenin signaling pathway

Fig. 2

EV-Wnt7b mRNA is highly expressed in HPV 16/18-positive CC cells and decrease by E6 knockdown. a Transmission electron microscopy and nanoparticle tracking analysis were used to evaluate CC-derived EVs. The EVs derived from CaSki, SiHa, HeLa and SW756 cells exhibited similar typical round morphologies, with particles ranging from 50 to 150 nm in diameter. b Western blot analysis showed positive expression of the known EV markers CD63, CD9, TSG101 and HSP 70 in EVs derived from CaSki, SiHa, HeLa or SW756 cells. c qRT-PCR analysis was used to assess Wnt7b in CC-derived EVs. Wnt7b mRNA levels were significantly increased in in EVs derived from 4 HPV 16/18-positive cell lines (the CaSki, SiHa, HeLa and SW756 cell lines) compared to EVs from HPV-negative C33A cells. d qRT-PCR analysis showed that E6 knockdown decreases Wnt7b mRNA loading in HPV 16/18 positive cell-derived EVs. * P < 0.05, ** P < 0.01

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