Fig. 4

Extracellular vesicular Wnt7b mediates the HPV 16/18 E6-induced proliferation and angiogenesis of HUVECs. a After the introduction of Wnt7b-OE into HPV 16 E6-KD SiHa and CaSki cells and HPV 18 E6-KD HeLa and SW756 cells, Western blot examination confirmed flag-tagged Wnt7b expression in HPV E6-KD + Wnt7b-OE cells. b A CCK8 proliferation assay was performed with HUVECs. HUVECs were separately cocultured with PBS, EV/NC, EV/E6-KD or EV/E6-KD + Wnt7b-OE derived from the 4 HPV 16/18-positive CC cell lines. c Tube formation by HUVECs was evaluated. HUVECs were separately cocultured with PBS, EV/NC, EV/E6-KD or EV/E6-KD + Wnt7b-OE derived from the 4 HPV 16/18-positive CC cell lines. Left: representative photographs illustrating the dynamics of the angiogenic behavior of HUVECs. Right: the relative tube lengths in HUVEC cultures. d HUVECs were cocultured with recombinant Wnt7b and the controls. The treatment with recombinant Wnt7b promoted HUVEC angiogenesis compared with the treatment of controls. * P < 0.05, ** P < 0.01